RNA Reagent 200ml 核酸萃取液
RNA Reagent 是即用型單相溶液，適合分離Total RNA，DNA和蛋白質。
1. 單一步驟從組織、細胞、細菌、植物、酵母菌
和生物體液中分離Total RNA 。
2. Total RNA 分離過程時間小於1個小時。
3. 純化的RNA可應
試劑是由酚，硫氰酸胍和緩衝液所配製而成的混合液，可從動物和植物組織、細胞和細菌培養液中分離總量核醣核酸、去氧核醣核酸和蛋白質。總量核醣核酸萃取整個過程是 Chomczynski 和 Sacchi 所發明。樣品在均質化和氯仿萃取之後形成三個液相層（上清液，中間和有機層）。核醣核酸存在上清液中可經由加入異丙醇沉澱，去氧核醣核酸可存在中層可經由酒精沉澱回收，蛋白質存在有機可經由層異丙醇沉澱出來。
Description
RNA REAGENT is a ready-to-use reagent for the isolation of total RNA from cells and tissues. Just a single-step is used during cell homogenization or lysate.1ml of reagent will treat small quantities of tissue (50-100 mg) or cells (5X 10 6 ) well.
Storage condition
2 to 8°C stable for 12 months
Application
RT-PCR
Northern hybridization
RNase protection d. poly-A+ RNA selection Differential display and microarray assay
Quality result
a variety of RNA species has an A260/A280 ratio 1.8 to 2.1when diluted into TE.