BioDiamond RT-PCR mix 混合酵素

• Description:

• RT-PCR Mix :

• Application

• Protocol

           1.Thaw the RT-PCR Mix at room temperature. Vortex the RT-PCR Mix and then spin it briefly in a micro centrifuge to collect the material in the bottom of the tube.
           2. Incubate the RNA 0.1-5 ug (optional add 2ul 5X RNA Protector) in RNase free ddH2O to final volume 10 ul and stand at 65°C for 15 min, and then keep on ice.
           3. Prepare one of the following reaction mixes(Table 1.) on ice.
           4. Incubate the mixture at 37-42°C during 30-120 min. The time of reaction depends on the length of cDNA, 30 min is for cDNA in range of 500 bp, 120 min is for cDNA                    more then 1.5 kb. The temperature of the reaction depends on the structural features of RNA. Use increased temperature (up to 50°C) for the highly structured RNA                       when treated 37-42°C for 10mins later.
           5. Heat the mixture 5 min at 80°C to inactivate the RTase.
           6. Use the mixture for PCR or for other application. For your PCR-Reaction you need 1-10 μl of your RT product.