RNA Reagent 200ml 核酸萃取液
RNA Reagent 是即用型单相溶液，适合分离Total RNA，DNA和蛋白质。
1. 单一步骤从组织、细胞、细菌、植物、酵母菌
和生物体液中分离Total RNA 。
2. Total RNA 分离过程时间小於1个小时。
3. 纯化的RNA可应
试剂是由酚，硫氰酸胍和缓冲液所配制而成的混合液，可从动物和植物组织、细胞和细菌培养液中分离总量核醣核酸、去氧核醣核酸和蛋白质。总量核醣核酸萃取整个过程是 Chomczynski 和 Sacchi 所发明。样品在均质化和氯仿萃取之后形成三个液相层（上清液，中间和有机层）。核醣核酸存在上清液中可经由加入异丙醇沉淀，去氧核醣核酸可存在中层可经由酒精沉淀回收，蛋白质存在有机可经由层异丙醇沉淀出来。
Description
RNA REAGENT is a ready-to-use reagent for the isolation of total RNA from cells and tissues. Just a single-step is used during cell homogenization or lysate.1ml of reagent will treat small quantities of tissue (50-100 mg) or cells (5X 10 6 ) well.
Storage condition
2 to 8°C stable for 12 months
Application
RT-PCR
Northern hybridization
RNase protection d. poly-A+ RNA selection Differential display and microarray assay
Quality result
a variety of RNA species has an A260/A280 ratio 1.8 to 2.1when diluted into TE.